Dimethyl Carbonate as a Mobile-Phase Modifier for Normal-Phase and Hydrophilic Interaction Liquid Chromatography

We studied the use of dimethyl carbonate (DMC) as a non-toxic, aprotic modifier for hydrophilic liquid interaction chromatography (HILIC) and normal-phase (LC). A comparison ethyl acetate (EA) DMC organic mobile-phase modifiers in hexane LC phthalates was conducted with silica column showed that retention factors (k) at same percentage were about factor two greater DMC. Detection 215 nm, possible DMC, allowed better detection by 10, compared EA detection, best 254 nm wavelength. Using core-shell column, HILIC separations trans-ferulic acid, syringic vanillic acid between acetonitrile (MeCN) portion mobile phase, from 80–95%. The analyte when to MeCN, 1.5 times greater, only moderate increase back pressure. Plate count peak asymmetry somewhat chromatograms, those MeCN. Seven mono- di-hydroxybenzoic positional isomers could be resolved effectively Sorbate benzoate preservatives commercial drinks also determined.